Generator

Part:BBa_K1783000

Designed by: Iowis Zhu   Group: iGEM15_UMaryland   (2015-06-03)


Miraculin Generator

pBAD-B0034-ExpTag-Linker-Miraculin-Linker-6xHis. Adds a promoter to BBa_K1033120 in order to characterize it. Under arabinose induction, produces miraculin connected to an export tag and a His-tag via unstructured linkers. Miraculin is a taste modifying protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None